ABSTRACT: Areas affected by coal mining can be recovered by revegetation with leguminous plants associated with nitrogen-fixing bacteria. This study addressed the isolation and characterization of native nitrogen-fixing bacteria from coalmine wasteland under different vegetation restoration approaches using Macroptilium atropurpureum (DC) Urb and Vicia sativa L. as trap plants. The bacteria were characterized and identified on the basis of 16S rRNA sequences. Additionally, nitrogen-fixing strains were characterized for tolerance to high heavy metal and low pH levels, as well as […]

Non-symbiotic diazotrophic bacteria (NSDB) influence plant growth through biological nitrogen fixation and also production and release of plant growth-regulating substances. The aim of this study was to evaluate the density and phenotypic diversity of this bacterial group in fragments of the “Reserva Biológica Serra dos Toledos” and surroundings, in Itajubá/MG. This reserve is located in the environmental protection area of Mantiqueira and represents an important recharge area and shelter for endemic flora and fauna. Topsoil samples were collected in areas […]

Grass species, which are frequently used for rehabilitation of degraded areas, can establish root associations with nitrogen fixing bacteria thus contributing to the ecosystem’s sustainability. On the other hand, microbial diversity plays an important role in the resilience of biological processes, including N2 fixation. This study aimed at the phenotypic characterization of 72 diazotrophic Gram-negative bacteria. Samples were isolated from areas under different rehabilitation strategies after bauxite mining in Poços de Caldas, Minas Gerais, Brazil, and inoculated in NFb, Fam, and […]

Diazotrophic endophytic bacteria enhance plant growth through biological nitrogen fixation and production and release of plant growth regulating substances, which facilitate the revegetation of areas degraded by human activities. However, little is known about populations of such bacteria in soils or plants of mining areas. Aiming to study the effects of different vegetation types and rehabilitation periods on some endophytic diazotrophic bacteria species, soil samples were collected under two environmental conditions (“Campo” and “Serra”) in bauxite mined areas undergoing different […]