Effects of Simulated Nitrogen Deposition and Precipitation Manipulation on Soil Microorganisms in the Desert Steppe of Northern China
Jia Meiqing, Huang Jing, Yang Yinghua, Han Guodong, Zhang Guogang 
07/Jan/2019
ABSTRACT: Soil microorganisms are influenced by climate change. However, the effect of climate change on soil cultivable bacteria are unclear. In this study, the composition and diversity of the soil cultivable bacterial community were explored by a dilution–plate method, PCR, and 16S rRNA sequencing in a desert steppe of northern China after repeated NH4NO3 amendments and precipitation manipulation for seven years. The experimental treatments were as follows: control (CK), N addition (+N), N addition plus water addition (+N+W), and N […]
Isolation, Characterization and Symbiotic Efficiency of Nitrogen-Fixing and Heavy Metal-Tolerant Bacteria from a Coalmine Wasteland
05/Sep/2018
ABSTRACT: Areas affected by coal mining can be recovered by revegetation with leguminous plants associated with nitrogen-fixing bacteria. This study addressed the isolation and characterization of native nitrogen-fixing bacteria from coalmine wasteland under different vegetation restoration approaches using Macroptilium atropurpureum (DC) Urb and Vicia sativa L. as trap plants. The bacteria were characterized and identified on the basis of 16S rRNA sequences. Additionally, nitrogen-fixing strains were characterized for tolerance to high heavy metal and low pH levels, as well as […]
Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation
01/Jun/2010
Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity […]
Microbial communities, enzymatic activity and mycorrhizal fungi in rhizospheric soil used for Landfarming of Petrochemical Waste
01/Aug/2008
The presence of plants stimulates soil microbiota, which may contribute to soil remediation. It is of great interest to quantify these effects on heterotrophic microorganisms due to their practical and ecological relevance. The following factors were evaluated: bacterial cell density, enzymatic activity, bacterial community structure and the spontaneous occurrence of arbuscular mycorrhizal fungi (AMF) in the plant rhizosphere in an area of land farming of petrochemical wastes, using a rhizospheric soil under five plants and control soil without plants. The […]