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All publications of “Maria Catarina Megumi Kasuya”

9 results

Effect of Inoculation of Pineapple Plantlets with Arbuscular Mycorrhizal Fungi Obtained from Different Inoculum Sources Multiplied by the On-Farm Method

Bruno Coutinho Moreira ORCID logo , Paulo Prates Junior ORCID logo , Thuany Cerqueira Jordão ORCID logo , Marliane de Cássia Soares da Silva ORCID logo , Ana Paula Ferreira Ribeiro ORCID logo , Sidney Luiz Stürmer ORCID logo , [...]


ABSTRACT The production of inoculum from arbuscular mycorrhizal fungi (AMF) at a large scale and low cost is essential for establishing methods to assist in producing pineapple plantlets with high nutritional and phytosanitary quality. However, this objective is difficult to accomplish because of the biotrophic nature of these fungi. The on-farm multiplication method for AMF inoculum presents a good alternative to supply the demand for the production of glomerospores. This study aimed to multiply and evaluate AMF inoculum originating from […]

Arbuscular mycorrhiza and plant tolerance to stress

Muriel da Silva Folli-Pereira, Lydice Sant'Anna Meira-Haddad, Denise Mara Soares Bazzolli, Maria Catarina Megumi Kasuya


Arbuscular mycorrhizal fungi (AMF) are soil fungi, obligate biotrophic fungi and form the most common mutualistic symbiosis in nature: the arbuscular mycorrhiza (AM). This association occurs on the roots of most plants, promoting improvements in plant growth and development and increasing tolerance and/or plant resistance to several adverse environmental agents. In addition, AMF can be a potential biological control agent of plant diseases. These fungi produce glomalin, a protein that plays a key role in soil stability and in the […]

Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp.

Marlon Corrêa Pereira, Nívea Moreira Vieira, Marcos Rogério Tótola, Maria Catarina Megumi Kasuya


Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated […]

Morphological and molecular characterization of Pisolithus in soil under eucalyptus plantations in Brazil

Maria Catarina Megumi Kasuya, Irene da Silva Coelho, Daniela Tiago da Silva Campos, Elza Fernandes de Araújo, Yutaka Tamai, Toshizumi Miyamoto


Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained […]

Diversity of mycorrhizal fungi Epulorhiza spp. isolated from Epidendrum secundum (Orchidaceae)

Marlon Corrêa Pereira, Olinto Liparini Pereira, Maurício Dutra Costa, Rodrigo Barros Rocha, Maria Catarina Megumi Kasuya


Rhizoctonia-like mycorrhizal fungi Epulorhiza spp. have been isolated from orchids of the genus Epidendrum and have been used to promote the symbiotic germination of orchid seeds. Epidendrum secundum is a widely distributed orchid in campo de altitude (high elevation grassy vegetation) regions of the State Park of Serra do Brigadeiro (PESB), Minas Gerais, Brazil, and little is known about the mycorrhizal relationships of this species in the park. The objective of this study was to evaluate the morphological diversity of […]

Comparision of methods to visualise arbuscular mycorrhizal and dark septate endophytic fungi in native Cerrado species

Kelly da Silva Coutinho Detmann, Marina Neves Delgado, Vitor Paiva Alcoforado Rebello, Tiago de Souza Leite, Aristéa Alves Azevedo, Maria Catarina Megumi Kasuya, [...]


Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi – plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can be determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and dark septate endophytic fungi (DSEF) in roots of […]

Compatibility and ectomycorrhiza formation among Pisolithus Isolates and Eucalyptus spp

Olinto Liparini Pereira, Maurício Dutra Costa, Arnaldo Chaer Borges, Elza Fernandes Araújo, Maria Catarina Megumi Kasuya


Twenty-nine isolates of the ectomycorrhiza fungus Pisolithus sp. from different geographical and host origins were tested for their ability to form ectomycorrhizae on Eucalyptus grandis and E. urophylla seedlings under greenhouse conditions. The ectomycorrhiza-forming capacity of isolates varied greatly from one eucalypt species to the other. All isolates from Eucalyptus, nine from Pinus spp. and two isolates from unknown hosts formed mycorrhizae with E. grandis and E. urophylla. Root colonization rates varied from 0 to 5.2 % for all Pinus isolates […]

Isolation and identification of rhizoctonia-like mycorrhizal fungi associated to three neotropical epiphytic orchid species in Brazil

Olinto Liparini Pereira, Maria Catarina Megumi Kasuya, Christtianno de Lima Rollemberg, Guilherme Montandon Chaer


Anthropogenic disturbances have resulted in an increased threat of extinction of many native orchid taxa in Brazil’s Atlantic rain forest. In nature, orchids utilize mycorrhizal fungi to initiate seed germination and seedling development, at least in the early stages of their life cycle. Mycorrhizal fungi associated with the roots of orchids have thus been isolated, characterized and stored as important resources for a future conservation program of orchid species through symbiotic seed germination. Three mycorrhizal Rhizoctonia-like fungi were isolated from […]

In vitro symbiotic seed germination of Oncidium flexuosum (Orchidaceae) by rhizoctonia-like mycorrhizal fungi

Olinto Liparini Pereira, Maria Catarina Megumi Kasuya, Christtianno de Lima Rollemberg, Arnaldo Chaer Borges


Symbiotic in vitro seed germination and protocorm development of Oncidium flexuosum is described for the first time. O. flexuosum seeds were inoculated with ten mycorrhizal isolates, originally obtained from the mycorrhiza of ten neotropical Brazilian orchid species, including O. flexuosum. One of the isolates belongs to Epulorhiza repens, two to Epulorhiza epiphytica, six to Ceratorhiza spp., and one to Rhizoctonia sp. Seeds inoculated with the M2 isolate of Ceratorhiza sp., originally isolated from the root system of wild O. flexuosum, […]